Evolution of the Bordetella autotransporter Pertactin: identifi cations of regions subject to positive selection
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Purification and Protective Efficacy of Re- Natured C-terminal Domain (p.30) of Pertactin Autotransporter Protein of Bordetella Pertussis
Members of the Bordetella pertussis autotransporter family are grouped together on the basis of homology at their C-termini. The full pathogenic potential of the B. pertussis is partly contributed by the members of its autotransporter family, which are usually either exported to the bacterial cell surface or secreted into the external environment. The β-barrel (C-terminus) forming domain plays ...
متن کاملPolymorphism of repeated regions of pertactin in Bordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica.
Pertactin is an outer membrane protein expressed by Bordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica that induces protective immunity to Bordetella infections. The immunodominant and immunoprotective epitopes of pertactin include two repeated regions, I and II. Comparison of these two repeated regions showed that B. parapertussis pertactin is invariant, whereas B. p...
متن کاملPertactin is required for Bordetella species to resist neutrophil-mediated clearance.
Pertactin (PRN) is an autotransporter protein produced by all members of the Bordetella bronchiseptica cluster, which includes B. pertussis, B. parapertussis, and B. bronchiseptica. It is a primary component of acellular pertussis vaccines, and anti-PRN antibody titers correlate with protection. In vitro studies have suggested that PRN functions as an adhesin and that an RGD motif located in th...
متن کاملPertactin Processing and Secretion in Protease(s) Deficient Strains of Escherichia Coli Suggestive of the Possible Role of Proteases in Folding of Autotransporter Proteins
Many of the virulence determinants secreted from pathogenic Gram-negative bacteria are autotransporter proteins, which are usually either exported to the bacterial cell surface or secreted into the external environment. There appears to be dearth of information regarding the exact mechanism of their processing into the surface-exposed passenger domains and C-terminal, βdomains. The C-terminal p...
متن کاملNovel genetic and phenotypic heterogeneity in Bordetella bronchiseptica pertactin.
The Bordetella bronchiseptica outer membrane protein pertactin is believed to function as an adhesin and is an important protective immunogen. Previous sequence analysis of the pertactin gene identified two regions predicted to encode amino acid repeat motifs. Recent studies have documented DNA sequence heterogeneity in both regions. The present study describes additional variants in these regi...
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